Avtor/Urednik		Hevir, Neli; Šinkovec, Jasna; Lanišnik-Rižner, Tea
Naslov		Disturbed expression of phases I and II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT
Tip		članek
Vir		Mol Cell Endocrinol
Vol. in št.		Letnik 331, št. 1
Leto izdaje		2011
Obseg		str. 158-67
Jezik		eng
Abstrakt		Expression levels of genes encoding phases I and II estrogen-metabolizing enzymes: CYP1A1, CYP1A2, CYP1B1, CYP3A5, CYP3A7, SULT1A1, SULT1E1, SULT2B1, COMT, UGT2B7, and GSTP1 were studied by real-time PCR in 38 samples of cancerous and adjacent control endometrium. We found significantly lower levels of CYP1B1 and CYP3A7, higher levels of SULT2B1, UGT2B7 and GSTP1, and no differences in expression of COMT, CYP1A1, CYP3A5, SULT1E1 and SULT2A1 in the endometrial cancers. The CYP1B1 and COMT proteins were also examined by Western blotting and immunohistochemical staining, supporting the real-time PCR analysis. Lower levels of CYP1B1 detected in cancerous endometrium suggest its important role in control, precancerous tissue. Additionally, we showed for the first time higher protein levels of soluble COMT in cancerous endometrium, and higher levels of membrane-bound COMT in control, precancerous endometrium. The importance of the changed ratio between soluble and membrane-bound COMT still needs to be evaluated in further studies.
Deskriptorji		ENDOMETRIAL NEOPLASMS ESTROGENS CYTOCHROME P-450 METHYLTRANSFERASES POLYMERASE CHAIN REACTION BLOTTING, WESTERN IMMUNOHISTOCHEMISTRY