Avtor/Urednik     Žiberna, Lovro; Lunder, Mojca; Može, Špela; Vanzo, Andreja; Tramer, Federica; Passamonti, Sabina; Drevenšek, Gorazd
Naslov     Acute cardioprotective and cardiotoxic effects of bilberry anthocyanins in ischemiar-reperfusion injury: beyond concentration-dependent antioxidant activity
Tip     članek
Vir     Cardiovasc Toxicol
Vol. in št.     Letnik 10, št. 4
Leto izdaje     2010
Obseg     str. 283-94
ISSN     1530-7905
Jezik     eng
Abstrakt     Despite being reported to reduce the risk of cardiovascular diseases, little is known about acute direct effects of bilberry anthocyanins on whole mammalian heart under ischemia-reperfusion (I-R) conditions. Bilberry anthocyanins were prepared from the ripe bilberries and analyzed using HPLC-DAD. Their antioxidant activity was evaluated by measuring the intrinsic free radical-scavenging capacity and by cellular antioxidant assay (CAA) on endothelial cells, where we quantified the intracellular capacity to inhibit the formation of peroxyl radicals. Experiments on the isolated rat hearts under I-R were carried out according to the Langendorff method. Perfusion with low concentrations of bilberry anthocyanins (0.01-1 mg/L) significantly attenuated the extent of I-R injury as evidenced by decreasing the release rate of LDH, increasing the postischemic coronary flow, and by decreasing the incidence and duration of reperfusion arrhythmias. High concentrations (5-50 mg/L) diminished cardioprotection and show cardiotoxic activity despite having their radical scavenging and intracellular antioxidant capabilities increased in a concentration-dependent manner. This study reveals the biphasic concentration-dependent bioactivity of bilberry anthocyanins under I-R, which results in strong cardioprotective activity in low concentrations and cardiotoxic activity in high concentrations.
Deskriptorji     ENDOTHELIUM
PLANT EXTRACTS
ANTHOCYANINS
FREE RADICAL SCAVENGERS
MYOCARDIAL REPERFUSION INJURY
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
CELL LINE
HEART RATE
ARRHYTHMIA
ELECTROCARDIOGRAPHY
LACTATE DEHYDROGENASE
RATS, WISTAR