Avtor/Urednik		Simončič, Matjaž; Režen, Tadeja; Juvan, Peter; Rozman, Damjana; Fazarinc, Gregor; Fievet, Catherine; Staels, Bart; Horvat, Simon
Naslov		Obesity resistant mechanisms in the Lean polygenic mouse model as indicated by liver transcriptome and expression of selected genes in skeletal muscle
Tip		članek
Vir		BMC Genomics
Vol. in št.		Letnik 12, št. 96
Leto izdaje		2011
Obseg		str. 1-38
Jezik		eng
Abstrakt		Background: Divergently selected Lean and Fat mouse lines represent unique models for a polygenic form of resistance and susceptibility to obesity development. Previous research on these lines focused mainly on obesity-susceptible factors in the Fat line. This study aimed to examine the molecular basis of obesity-resistant mechanisms in the Lean line by analyzing various fat depots and organs, the liver transcriptome of selected metabolic pathways, plasma and lipid homeostasis and expression of selected skeletal muscle genes. Results: Expression profiling using our custom Steroltalk v2 microarray demonstrated that Lean mice exhibit a higher hepatic expression of cholesterol biosynthesis genes compared to the Fat line, although this was not reflected in elevation of total plasma or liver cholesterol. However, FPLC analysis showed that protective HDL cholesterol was elevated in Lean mice. A significant difference between the strains was also found in bile acid metabolism. Lean mice had a higher expression of Cyp8b1, a regulatory enzyme of bile acid synthesis, and the Abcb11 bile acid transporter gene responsible for export of acids to the bile. Additionally, a higher content of blood circulating bile acids was observed in Lean mice. Elevated HDL and upregulation of some bile acids synthesis and transport genes suggests enhanced reverse cholesterol transport in the Lean line - the flux of cholesterol out of the body is higher which is compensated by upregulation of endogenous cholesterol biosynthesis. Increased skeletal muscle Il6 and Dio2 mRNA levels as well as increased activity of muscle succinic acid dehydrogenase (SDH) in the Lean mice demonstrates for the first time that changes in muscle energy metabolism play important role in the Lean line phenotype determination and corroborate our previous findings of increased physical activity and thermogenesis in this line. (Abstract truncated at 2000 characters)
Deskriptorji		OBESITY CHOLESTEROL LIPOPROTEINS, HDL CHOLESTEROL BILE ACIDS AND SALTS OLIGONUCLEOTIDE PROBES MUSCLE, SKELETAL SUCCINATE DEHYDROGENASE PHENOTYPE ENERGY METABOLISM DISEASE MODELS, ANIMAL MICE, INBRED STRAINS