Avtor/Urednik		Boehm, Bernd; Rautschka, Michael; Quintana, Laura; Raspopovic, Jelena; Jan, Žiga; Sharpe, James
Naslov		A landmark-free morphometric staging system for the mouse limb bud
Tip		članek
Vir		Dev Suppl
Vol. in št.		Letnik 138, št. 6
Leto izdaje		2011
Obseg		str. 1227-34
Jezik		eng
Abstrakt		We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about +/-2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and thereby stage the bud within a couple of minutes. We describe how the system is constructed, its robustness to user variation and illustrate one application: the accurate tracking of spatiotemporal dynamics of gene expression patterns.
Deskriptorji		LIMB BUD BIOMETRY GESTATIONAL AGE GENE EXPRESSION GASTRULA COMPUTER SIMULATION IMAGE PROCESSING, COMPUTER-ASSISTED MICE, INBRED CBA MICE, INBRED C57BL