Avtor/Urednik		Banič, Blaž; Nipič, Damijan; Šuput, Dušan; Milisav, Irina
Naslov		DMSO modulates the pathway of apoptosis triggering
Tip		članek
Vir		Cell Mol Biol Lett
Vol. in št.		Letnik 16, št. 2
Leto izdaje		2011
Obseg		str. 328-41
Jezik		eng
Abstrakt		We demonstrate here that distribution of caspase-9 influences the pathway of apoptosis triggering, since caspase-9 is activated efficiently only when it is distributed solely in the cytosol. Caspase-9 moves to the nuclei in a response to cell stress during isolation of primary hepatocytes; this is called preapoptotic cell stress response. The dimethyl sulfoxide (DMSO) treatment cannot prevent the migration of caspase-9 into the nuclei when it is added to primary hepatocytes immediately after isolation; however, it can trigger redistribution of caspase-9 from the nuclei into the cytosol when added 1 day post-isolation. This redistribution is temporary, since caspase-9 returns to the nuclei within 48 hours of DMSO treatment. Thereafter, some caspase-9 is retained in the nuclei of DMSO-treated hepatocytes for longer than in the nuclei of untreated hepatocytes. By measuring caspase activities, we demonstrate that the addition of DMSO to cell culture medium can temporarily normalize the susceptibility of hepatocytes for apoptosis triggering through the intrinsic pathway. DMSO contributes also to the prolonged pathway inactivation, i.e., by extending preapoptotic cell stress response. We propose that DMSO extends the survival of primary hepatocytes by modulating preapoptotic cell stress response, which could be exploited for extending the lifespan of other primary cell cultures.
Deskriptorji		LIVER DIMETHYL SULFOXIDE CYSTEINE PROTEINASES CYTOSOL APOPTOSIS IMMUNOHISTOCHEMISTRY MICROSCOPY, CONFOCAL CELLS, CULTURED RATS, WISTAR