Avtor/Urednik		Seme, Katja; Močilnik, Tina; Poljak, Mario
Naslov		Twenty-four mini-pool HCV RNA screening in a routine clinical virology laboratory setting: a six-year prospective study
Tip		članek
Vir		J Virol Methods
Vol. in št.		Letnik 171, št. 1
Leto izdaje		2011
Obseg		str. 303-5
Jezik		eng
Abstrakt		The usefulness of combined anti-HCV and 24 mini-pool HCV RNA screening strategy was re-evaluated after a six-year continuous routine use in a clinical virology laboratory, at which more than half of newly diagnosed hepatitis C patients are intravenous drug users. Pools of 24 samples were prepared from 20,448 anti-HCV negative serum samples and tested using an automated commercial PCR assay with a lower limit of detection of 50 IU/ml. After detection of anti-HCV negative/HCV RNA positive patients, responsible physicians provided follow-up samples. Thirty-eight (0.19%) anti-HCV negative/HCV RNA positive samples from 30 patients (28 intravenous drug users) were detected. Follow-up samples were available for 27/30 patients. Twenty, six and one patient seroconverted in the second, third and fourth available samples, respectively. The interval between the first HCV RNA positive and the first available anti-HCV positive sample was 17-517 days. The costs of detecting a single anti-HCV negative/HCV RNA positive patient were 1227 Euros. Combined anti-HCV and 24 mini-pool HCV RNA screening is a useful and cost effective strategy, not only in blood-transfusion settings but also in a routine clinical virology laboratory, at which a significant proportion of the tested population belongs to a high-risk population.
Deskriptorji		HEPATITIS C HEPATITIS C-LIKE VIRUSES HEPATITIS C ANTIBODIES RNA, VIRAL SUBSTANCE ABUSE, INTRAVENOUS PROSPECTIVE STUDIES