Avtor/Urednik | Velebit, Jelena; Chowdhury-Haque, Helena; Kreft, Marko; Zorec, Robert | |
Naslov | Rosiglitazone balances insulin-induced exo- and endocytosis in single 3T3-L1 adipocytes | |
Tip | članek | |
Vir | Mol Cell Endocrinol | |
Vol. in št. | Letnik 333, št. 1 | |
Leto izdaje | 2011 | |
Obseg | str. 70-7 | |
Jezik | eng | |
Abstrakt | Rosiglitazone (Rosi) improves insulin sensitivity and increases the translocation of glucose transporter 4 (GLUT4) to the plasma membrane (PM). This involves the fusion of membrane-bound compartments with the plasma membrane, thus increasing the plasma membrane area. However, recent work has shown that in Rosi-pretreated 3T3-L1 adipocytes membrane area did not increase following insulin application, suggesting that the rates of exo- and endocytosis are balanced. Here we examined whether Rosi differentially affects the rates of exo- and endocytosis in 3T3-L1 adipocytes. The immunolabelling of GLUT4 revealed the 3.1-fold increase in PM-resident GLUT4 in Rosi-pretreated, insulin-stimulated cells. By monitoring cumulative exocytosis and endocytosis we found that in Rosi-pretreated cells insulin substantially stimulated the rate of exocytosis and to a similar extent also the rate of endocytosis. We conclude that Rosi-pretreatment balances insulin-stimulated exocytosis and endocytosis, which may prevent insulin-mediated adipocyte cell size increase. | |
Deskriptorji | ADIPOCYTES INSULIN EXOCYTOSIS ENDOCYTOSIS MONOSACCHARIDE TRANSPORT PROTEINS CELL SIZE FLUORESCENT ANTIBODY TECHNIQUE IMMUNOHISTOCHEMISTRY CELLS, CULTURED MICROSCOPY, CONFOCAL RATS |