Avtor/Urednik		Ružić-Sabljić, E
Naslov		Detection of Borrelia burgdorferi by polymerase chain reaction in the biological fluids
Tip		članek
Vir		Acta Dermatovenerol Alp Pannon Adriat
Vol. in št.		Letnik 3, št. 1-2
Leto izdaje		1994
Obseg		str. 105-9
Jezik		eng
Abstrakt		Low bacterial loads and difficult growth in culture, slow antibody response, persistence of specific antibody response even after asymptomatic infections and negative results of serological tests are characteristic for B. burgdorferi infection. To facilitate the understanding of B. burgdorferi infection the PCR has been applied as a method capable of elucidating the pathogenesis of infection and as additional method of confirming borrelial infection. The PCR is a method for amplifying specific nucleic acid sequences by use of repeated cycles of DNA synthesis. Under appropriate conditions, the PCR can be used to identify B. burgdorferi in human tissues and biological fluids; less than 10 microorganisms in samples can be identified. The specificity is determined by the choise of an optimal DNA sequence of B. burgdorferi as a target sequence for the amplification. Applicability of the PCR for routine diagnostic procedure should be established by more detailed investigations.
Deskriptorji		BORRELIA BURGDORFERI POLYMERASE CHAIN REACTION LYME DISEASE BODY FLUIDS