Avtor/Urednik		Serša, G; Čemažar, M; Šentjurc, M; Us-Krašovec, M; Kalebić, S; Drašlar, K; Auersperg, M
Naslov		Effects of vinblastine on cell membrane fluidity and the growth of SA-1 tumor in mice
Tip		članek
Vir		Cancer Lett
Vol. in št.		Letnik 79, št. 1
Leto izdaje		1994
Obseg		str. 53-60
Jezik		eng
Abstrakt		Cell membranes can be targets of some anti-cancer drugs. Therefore, the purpose of this study was to determine whether vinblastine (VLB) can also affect the tumor cell membrane. On the in vivo SA-1 tumor model, alteration of cell membrane fluidity (measured by electron paramagnetic resonance, EPR), cytotoxicity and morphological changes of the SA-1 tumor cells after VLB treatment were studied. The cytotoxic effect of VLB was biphasic, with an initial fast increase in cytotoxicity followed by a plateau. The surviving cells had increased membrane fluidity and were morphologically changed. The dose-response curve of VLB on membrane fluidity was also biphasic with an initial fast increase in membrane fluidity followed by a plateau. Since dose-response curves of VLB cytotoxicity and its effect on membrane fluidity were similar, there was a high correlation between both effects. The effect of VLB on membrane fluidity was the most pronounced at 24 h and 48 h after treatment. The results of this study indicate that VLB affects cell membrane by increasing the membrane fluidity of SA-1 tumor cells in vivo in a dose-and time-dependent manner. Therefore, this finding may be beneficially implemented also in priming cells for other cytotoxic drugs and for appropriate timing of drug sequence in combined schedules.
Deskriptorji		FIBROSARCOMA MEMBRANE FLUIDITY SARCOMA, EXPERIMENTAL VINBLASTINE CELL DIVISION CELL MEMBRANE CELL SURVIVAL DOSE-RESPONSE RELATIONSHIP, DRUG ELECTRON SPIN RESONANCE SPECTROSCOPY FIBROSARCOMA MICE MICE, INBRED A MICROSCOPY, ELECTRON, SCANNING SARCOMA, EXPERIMENTAL TIME FACTORS