Avtor/Urednik		Maček, V; Šorli, J; Kopriva, S; Marin, J
Naslov		Persistent adenoviral infection and chronic airway obstruction in children
Tip		članek
Vir		Am J Respir Crit Care Med
Vol. in št.		Letnik 150, št. 1
Leto izdaje		1994
Obseg		str. 7-10
Jezik		eng
Abstrakt		Previous studies from several laboratories have established that adenovirus is a common cause of severe childhood bronchiolitis. The observation that children with an established history of bronchiolitis subsequently developed unremitting airways obstruction even after adequate steroid therapy led us to postulate that this bronchial obstruction might be due to persistence of an adenoviral infection. This hypothesis was tested by performing bronchoalveolar lavage (BAL) on a group of 34 children with a mean age of 5 yr (range, 14 mo to 14 yr) who showed an unfavorable response to standard corticosteroid and bronchodilator therapy. Analysis of cytospin preparations of BAL fluid at the light-microscopic level, using a monoclonal antibody to detect adenoviral antigens, demonstrated that capsid protein was present in 31 of 34 (94 percent) of the children examined. Limited repeat studies within 1 yr showed 6 of 8 (75 percent) were positive twice when tested on two occasions, and that three were positive in all occasions when sampled three times. Cultures of the BAL fluid were also positive for adenovirus in six of six cultures performed, indicating that the virus was in some cases replicating. Similar studies of control patients without persistent asthma showed no evidence of adenovirus. We conclude that persistent and/or latent adenoviral infection may contribute to the pathogenesis of childhood asthma in which there is an unfavorable response to steroid and bronchodilatation therapy.
Deskriptorji		ADENOVIRUS INFECTIONS, HUMAN ASTHMA BRONCHIOLITIS, VIRAL ASTHMA BRONCHOALVEOLAR LAVAGE FLUID CAPSID CHILD CHILD, PRESCHOOL CHRONIC DISEASE FLUORESCENT ANTIBODY TECHNIQUE INFANT PEAK EXPIRATORY FLOW RATE ADENOVIRUSES, HUMAN ADOLESCENCE ANTIGENS, VIRAL