Avtor/Urednik		Hudoklin, Samo; Jezernik, Kristijan; Neumuller, Josef; Pavelka, Marget; Romih, Rok
Naslov		Urothelial plaque formation in post-Golgi compartments
Tip		članek
Vir		PLoS One
Vol. in št.		Letnik 6, št. 8
Leto izdaje		2011
Obseg		str. e23636
Jezik		eng
Abstrakt		Urothelial plaques are specialized membrane domains in urothelial superficial (umbrella) cells, composed of highly ordered uroplakin particles. We investigated membrane compartments involved in the formation of urothelial plaques in mouse umbrella cells. The Golgi apparatus did not contain uroplakins organized into plaques. In the post-Golgi region, three distinct membrane compartments containing uroplakins were characterized: i) Small rounded vesicles, located close to the Golgi apparatus, were labelled weakly with anti-uroplakin antibodies and they possessed no plaques; we termed them uroplakin-positive transporting vesicles (UPTVs). ii) Spherical-to-flattened vesicles, termed immature fusiform vesicles (iFVs), were uroplakin-positive in their central regions and contained small urothelial plaques. iii) Flattened mature fusiform vesicles (mFVs) contained large plaques, which were densely labelled with anti-uroplakin antibodies. Endoytotic marker horseradish peroxidase was not found in these post-Golgi compartments. We propose a detailed model of de novo urothelial plaque formation in post-Golgi compartments: UPTVs carrying individual 16-nm particles detach from the Golgi apparatus and subsequently fuse into iFV. Concentration of 16-nm particles into plaques and removal of uroplakin-negative membranes takes place in iFVs. With additional fusions and buddings, iFVs mature into mFVs, each carrying two urothelial plaques toward the apical surface of the umbrella cell.
Deskriptorji		BLADDER UROTHELIUM CELL COMPARTMENTATION CELL MEMBRANE GOLGI APPARATUS MICROSCOPY, IMMUNOELECTRON FLUORESCENT ANTIBODY TECHNIQUE HORSERADISH PEROXIDASE FREEZE FRACTURING MICE, INBRED C57BL