Avtor/Urednik		Lovšin, Nika; Peterlin, Matija B
Naslov		APOBEC3 proteins inhibit LINE-1 retrotransposition in the absence of ORF1p binding
Tip		članek
Vir		Ann N Y Acad Sci
Vol. in št.		Letnik 1178
Leto izdaje		2009
Obseg		str. 268-75
Jezik		eng
Abstrakt		Members of the apolipoprotein B mRNA editing complex polypeptide 1-like (APOBEC) family of enzymes exhibit inhibitory activity against a variety of exogenous and endogenous retroviruses including retrotransposons, such as long interspersed element 1 (LINE-1). Indeed, human APOBEC3A, APOBEC3B, and APOBEC3F inhibit retrotransposition of human LINE-1, mouse IAP and MusD retrotransposons. In our study, we examined whether the inhibitory effect of APOBEC3 proteins correlates with APOBEC3 ability to bind the LINE-1 ORF1 protein. We examined the interactions between the LINE-1 ORF1 protein and the most potent LINE-1 retrotransposon inhibitors, human APOBEC3A and APOBEC3B, by immunofluorescence and immunoprecipitation. Although human APOBEC3A shows the highest inhibitory potency against LINE-1 retrotransposon, no direct interactions were identified either by immunofluorescence or by co-immunoprecipitation. APOBEC3B binds to LINE-1 ORF1 protein, yet no co-localization was detected. We concluded that APOBEC3 proteins interfere indirectly with the LINE-1 retrotransposition pathway, probably through interference with RNA targeting.
Deskriptorji		ANIMALS BINDING SITES CYTIDINE DEAMINASE FLUORESCENT ANTIBODY TECHNIQUE HELA CELLS MICE OPEN READING FRAMES TRANSFECTION