Avtor/Urednik		Žitnik, Simona EK; Vesel, Tina; Avčin, Tadej; Šilar, Mira; Košnik, Mitja; Korošec, Peter
Naslov		Monitoring honeybee venom immunotherapy in children with the basophil activation test
Tip		članek
Vir		Pediatr Allergy Immunol
Vol. in št.		Letnik 23, št. 2
Leto izdaje		2012
Obseg		str. 167-72
Jezik		eng
Abstrakt		Background: New in vitro methods are essential for developing better follow-up criteria for venom immunotherapy (VIT). Methods: Thirty-one children with a history of honeybee venom-induced systemic anaphylaxis were included in this prospective, single-blinded study. The basophil CD63 activation test (BAT) was assessed before starting VIT, at the end of the build-up phase (day 5), 6 months later, and after 2-4 yr of VIT. Results: Basophil CD63 activation test allowed identification of the culprit insect in 74% of honeybee venom-allergic children. In comparison, IgE reactivity was single positive in only 52% of children. Five days after starting VIT, BAT was highly comparable to before VIT. However, after 6 months and further after 2-4 yr of VIT, a significant and approximately fourfold decrease was demonstrated in CD63 response at sub-maximal 0.1 mug/ml allergen concentration, which mainly represents cellular sensitivity. No such differences were found at a higher 1 mug/ml of allergen concentration. Person-to-person analyses showed that after 2-4 yr of VIT, a marked CD63 decrease was evident in 85% of children. In addition, elevated basophil sensitivity measured before VIT was associated with the appearance of side effects observed during the build-up phase of VIT. Conclusion: Basophil CD63 allergen-specific sensitivity seems to be a promising tool for monitoring protective immune response in honeybee VIT.
Deskriptorji		BEE VENOMS ANAPHYLAXIS BASOPHILS BASOPHIL DEGRANULATION TEST IGE CHILD DESENSITIZATION, IMMUNOLOGIC PROSPECTIVE STUDIES CHILD