Avtor/Urednik		Vidovič-Valentinčič, Nataša; de Groot-Mijnes, Jolanda DF; Kraut, Aleksandra; Korošec, Peter; Hawlina, Marko; Rothova, Aniki
Naslov		Intraocular and serum cytokine profiles in patients with intermediate uveitis
Tip		članek
Vir		Mol Vis
Vol. in št.		Letnik 17
Leto izdaje		2011
Obseg		str. 2003-10
Jezik		eng
Abstrakt		Purpose: To study the intraocular and serum cytokine and chemokine profile in patients with intermediate uveitis (IU) at various stages of inflammatory activity. Methods: Institutional, prospective association study. Paired aqueous humor (AqH) and serum samples were collected from 36 consecutive IU patients and 10 controls. The concentrations of interleukin (IL)-1beta, IL-6, IL-8, IL-10, IL-12p70, tumor necrosis factor (TNF)-alpha, CC--chemokine ligand 5/regulated upon activation normal T-cell expressed, and secreted (CCL5/RANTES), CC--chemokine ligand 3/macrophage inflammatory protein 1alpha (CCL3/MIP-1alpha), CCL4/MIP-1beta, and CC--chemokine ligand 2/monocyte chemotactic protein--1 (CCL2/MCP-1) were measured in both AqH and serum by multiplex immunoassay. Main outcome measures were serum and intraocular levels of the analyzed cyto- and chemokines. Results: Patients with IU had higher serum levels of TNF-alpha than non-uveitic controls (p<0.0001), whereas their AqH TNF-alpha levels did not show a difference (p=0.323). IU patients had higher intraocular levels of IL-1beta, IL-6, IL-8, IL-10, IL-12p70 and CCL2/MCP-1 than the controls (p=0.020, 0.001, <0.0001, 0.005, 0.003, and 0.003, respectively). Active stages of IU were characterized by higher levels of IL-6, IL-8, CCL5/RANTES and CCL2/MCP-1 (p=0.003, <0.0001, 0.033, and 0.033, respectively). Higher levels of IL-6 and IL-8 were found in IU patients with cystoid macular edema (CME) compared to non-CME IU patients (p=0.026 and 0.012, respectively). Significant positive correlations between various observed mediators were present in the AqH of IU patients only. Conclusions: Significantly elevated concentrations of multiple intraocular cytokines were found in IU patients, especially IL-6 and IL-8 in those with CME and active disease. In serum elevated TNF-alpha levels were observed in IU patients. (Abstract truncated at 2000 characters)
Deskriptorji		UVEITIS, INTERMEDIATE CYTOKINES AQUEOUS HUMOR INTERLEUKIN-1 INTERLEUKIN-6 INTERLEUKIN-8 INTERLEUKIN-10 INTERLEUKIN-12 MACROPHAGE INFLAMMATORY PROTEIN-1 MONOCYTE CHEMOATTRACTANT PROTEIN-1 ADULT CASE-CONTROL STUDIES PROSPECTIVE STUDIES TUMOR NECROSIS FACTOR