Avtor/Urednik		Milisav, Irina; Šuput, Dušan
Naslov		Liver slices are the optimal model for mimicking apoptosis activation in vitro
Tip		članek
Vir		HealthMed
Vol. in št.		Letnik 5, št. 6 Suppl 1
Leto izdaje		2011
Obseg		str. 2047-50
Jezik		eng
Abstrakt		Tissue slicing enables sectioning of tissue with metabolically active cells. The cutting of untreated rat and human liver tissues has been optimized recently to produce uniformly thick tissue slices of 200 microm. There are no reports in the literature about the optimal slice thickness to study induction of apoptosis. Therefore, the objective of our study was to determine the optimal slice thickness that would enable performing a uniform pharmacological treatment as well as reproducible slicing. The native slices of liver from 80 microm to 200 microm are suitable for studies of biochemical pathways and metabolism, however, only the slices of 80 microm are a reliable model for apoptosis triggering in liver. These slices are the best model for studying apoptosis initiation in liver, since the pathways of apoptosis triggering are changed in the primary liver cells (hepatocytes) due to stress during their isolation. Tissue slices are important in vitro models for studying metabolism and may become useful for evaluation of medical procedures in cell therapies and regenerative medicine.
Deskriptorji		LIVER TISSUE CULTURE CYSTEINE PROTEINASES CULTURE MEDIA STREPTOMYCIN RATS, WISTAR