Avtor/Urednik     Xing, Wei; Akopian, Abram; Križaj, David
Naslov     Trafficking of presynaptic PMCA signaling complexes in mouse photoreceptors requires Cav1.4 alpha(1) Subunits
Tip     članek
Vir     Adv Exp Med Biol
Vol. in št.     Letnik 723, št. 9
Leto izdaje     2012
Obseg     str. 739-44
Jezik     eng
Abstrakt     The pore-forming Cav1.4 ?1 subunit of the L-type Ca2+ channel plays a critical role in voltage-dependent Ca2+ influx and transmitter release at mammalian photoreceptor and bipolar synapses. Here we ask whether loss of Cav1.4 protein in the nob2 ('no b-wave') Cacna1f mutation affects expression and localization of Ca2+ extrusion machinery at retinal ribbon synapses. Analysis of PMCA1, PMCA2, and pan-PMCA immunoreactivity in nob2 retinas showed pronounced mislocalization of presynaptic proteins in rod photoreceptors but not in bipolar neurons. PMCA1 transporters were displaced into juxtanuclear pockets across the outer nuclear layer (ONL) whereas little change was observed in localization of the high affinity Ca2+ extruder PMCA2. Our results therefore implicate Cav1.4 subunits in proper development and trafficking of signaling complexes to the photoreceptor synapse.
Deskriptorji     PORINS
CALCIUM
PHOTORECEPTORS
SYNAPSES
SYNAPTIC TRANSMISSION
RETINA
MUTATION
SIGNAL TRANSDUCTION
GENE EXPRESSION
MICE