Avtor/Urednik		Prijic, Sara; Prosen, Lara; Čemažar, Maja; Ščančar, Janez; Romih, Rok; Lavrenčak, Jaka; Bregar, Vladimir B; Coer, Andrej; Kržan, Mojca; Žnidaršič, Andrej; Serša, Gregor
Naslov		Surface modified magnetic nanoparticles for immuno-gene therapy of murine mammary adenocarcinoma
Tip		članek
Vir		Biomaterials
Vol. in št.		Letnik 33, št. 17
Leto izdaje		2012
Obseg		str. 4379-91
Jezik		eng
Abstrakt		Cancer immuno-gene therapy is an introduction of nucleic acids encoding immunostimulatory proteins, such as cytokine interleukin 12 (IL-12), into somatic cells to stimulate an immune response against a tumor. Various methods can be used for the introduction of nucleic acids into cells; magnetofection involves binding of nucleic acids to magnetic nanoparticles with subsequent exposure to an external magnetic field. Here we show that surface modified superparamagnetic iron oxide nanoparticles (SPIONs) with a combination of polyacrylic acid (PAA) and polyethylenimine (PEI) (SPIONs-PAA-PEI) proved to be safe and effective for magnetofection of cells and tumors in mice. Magnetofection of cells with plasmid DNA encoding reporter gene using SPIONs-PAA-PEI was superior in transfection efficiency to commercially available SPIONs. Magnetofection of murine mammary adenocarcinoma with plasmid DNA encoding IL-12 using SPIONs-PAA-PEI resulted in significant antitumor effect and could be further refined for cancer immuno-gene therapy.
Deskriptorji		MAMMARY NEOPLASMS, EXPERIMENTAL ADENOCARCINOMA GENE THERAPY MAGNETICS MICROCHEMISTRY GENE TRANSFER POLYETHYLENEIMINE TRANSFECTION ACRYLATES MICE, INBRED STRAINS