Avtor/Urednik		Erman, Andreja; Resnik, Nataša; Romih, Rok
Naslov		Autophagic activity in the mouse urinary bladder urothelium as a response to starvation
Tip		članek
Vir		Protoplasma
Vol. in št.		Letnik 250, št. 1
Leto izdaje		2013
Obseg		str. 151-60
Jezik		eng
Abstrakt		The urinary bladder urothelium is subjected to mechanical forces during cycles of distension and contraction, and its superficial cells are constantly flushed by toxic urine. Yet, the urothelium shows a very slow turnover of cells and superficial cells are extremely long lived. Autophagy has a well-known role in tissue homeostasis and serves as a protective mechanism against cellular stress. Therefore, the presence of autophagy as one of possible processes of survival in an unpleasant environment and during long lifetime of superficial cells was examined in mouse urothelium. We detected and evaluated autophagic activity of superficial urothelial cells under normal and stress conditions, caused by short-term starvation of newborn and 24-h-starved adult mice. Immunolabeling and Western blotting of essential effectors of autophagy, LC3 and Beclin 1, showed a weak signal in superficial urothelial cells. On the other hand, ultrastructural analysis, which proved to be the most reliable method in our study, revealed the presence of autophagic vacuoles, some of them containing specific urothelial structures, fusiform vesicles. Quantitative analysis showed increased autophagy in newborn and starved mice in comparison to a low basic level of autophagy in the urothelium of normal mice. Interestingly, some superficial cells of adults and neonates exhibit intense immunoreactions against LC3 and Beclin 1 and the typical ultrastructural characteristics of autophagy-dependent cell death. We conclude that autophagy, despite low basic activity under physiological conditions, plays an important role in urothelial homeostasis and stability under stress.
Deskriptorji		BLADDER UROTHELIUM STARVATION AUTOPHAGOCYTOSIS CELL DEATH BLOTTING, WESTERN IMMUNOHISTOCHEMISTRY MICROSCOPY, ELECTRON MICE, INBRED C57BL