Avtor/Urednik		Kočevar, Nina; Odreman, Federico; Vindigni, Alessandro; Frković-Grazio, Snježana; Komel, Radovan
Naslov		Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers
Tip		članek
Vir		World J Gastroenterol
Vol. in št.		Letnik 18, št. 11
Leto izdaje		2012
Obseg		str. 1216-28
Jezik		eng
Abstrakt		AIM: To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma. METHODS: We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting. RESULTS: We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border. CONCLUSION: This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis.
Deskriptorji		STOMACH NEOPLASMS ADENOCARCINOMA NEOPLASM PROTEINS ELECTROPHORESIS, GEL, TWO-DIMENSIONAL SPECTRUM ANALYSIS, MASS IMMUNOBLOTTING TUMOR MARKERS, BIOLOGICAL IMAGE PROCESSING, COMPUTER-ASSISTED