Avtor/Urednik		Jukić, Tomislav; Ihan, Alojz; Jukić, Dubravko
Naslov		Tetrahydrophthalazine derivative 'sodium nucleinate' exert its anti-Inflammatory effects through Inhibition of oxidative burst in human monocytes
Prevedeni naslov		Tetrahidroftalazinski derivat natrij nukleinat ispoljava protuupalno djelovanje kroz inhibiciju oksidativne aktivnosti humanih monocita
Tip		članek
Vir		Coll Antropol
Vol. in št.		Letnik 36, št. 2
Leto izdaje		2012
Obseg		str. 409-12
Jezik		eng
Abstrakt		We described the use of a new chemical substance Sodium nucleinate (SN) as an immunomodulatory substance exhibiting antiinflammatory properties. Sodium nucleinate (SN) registrated in Russian Federation as Tamerit®, is 2-amino-1,2,3,4-tetrahydrophthalazine-1,4-dione sodium salt dihydrate, derivative of well known chemical substance luminol. To comprehend the mechanisms of SN immunomodulatory activity, we examined the SN modulation of the oxidative burst responses of whole blood human monocytes and polimorphonuclear cells (PMC) stimulated with phorbol 12-myristate 13-acetate (PMA) or E. coli suspension in vitro. SN did not inhibit the proportion of neutrophils and monocytes phagocytosing E. coli. Oxidative burst responses of monocytes stimulated with PMA were strongly inhibited at SN concentration ranging from 10-500 mg/ml, less efficient inhibitor was SN in E. coli stimulated monocytes (inhibition range was from 50-500 mg/ml SN). SN inhibited PMC oxidative burst only in range 100-500 mg/ml SN. In conclusion, we found SN as an efficient inhibitor of oxidative burst in monocytes. Since ROS generation in monocytes/macrophages has been found to be important for LPS-driven production of several proinflammatory cytokines, SN may exsert its antiinflammatory effects through monocyte/macrophage oxidative burst inhibition.
Deskriptorji		MONOCYTES GRANULOCYTES PHTHALAZINES RESPIRATORY BURST ADJUVANTS, IMMUNOLOGIC