Avtor/Urednik		Vanhomwegen, Jessica; Alves, Maria Joao; Avšič-Županc, Tatjana; Korva, Miša; Saksida, Ana
Naslov		Diagnostic assays for crimean-Congo hemorrhagic fever
Tip		članek
Vir		Emerg Infect Dis
Vol. in št.		Letnik 18, št. 12
Leto izdaje		2012
Obseg		str. 1958-65
Jezik		eng
Abstrakt		Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofl uorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, inhouse methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specifi city was excellent for all assays; molecular test results were infl uenced by patient country of origin. Our fi ndings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
Deskriptorji		HEMORRHAGIC FEVER, CRIMEAN HEMORRHAGIC FEVER VIRUS, CRIMEAN-CONGO ENZYME-LINKED IMMUNOSORBENT ASSAY FLUORESCENT ANTIBODY TECHNIQUE POLYMERASE CHAIN REACTION IGG GENOME, VIRAL