Avtor/Urednik		Wraber, B
Naslov		Modulation of cytokine synthesis in human mononuclear cell cultures of different origin
Prevedeni naslov		Modulacija sinteze citokinov v kulturah humanih mononuklearnih celic različnega izvora
Tip		članek
Vir		Zdrav Vestn
Vol. in št.		Letnik 63, št. Suppl 2
Leto izdaje		1994
Obseg		str. II-57-II-6
Jezik		eng
Abstrakt		Background. Cytokines are small, secreted protein mediaton that act as intercellular communicators between various cells of the immune system as well as between these and other cells ofčthe organism. They, are mostly synthesized and secreted by the activated cells of the immune system. Cytokine synthesis is regulated by, differentiation of cells into various cytokine secreting phenotypes, and by selective activation of different types of cells to produce their characteristic cytokines. From the point of view that they, are natural immune response modifiers it is very important to know how the regulation of their synthesis and secretion occurs. Especially of interest are the differences between normal and by disease or therapy altered immune system. Due to the complexity of the cytokine network, it is difficult to follow and influence cytokine production in vivo, therefore, in vitro approach to this problem is much more frequently presented. Methods. The author studied modifications of the production of interleukin-1 alpha (Il-1 alpha), interleukin-1 beta (Il-1 beta) and interleukin (Il-2) in human peripheral blood mononuclear cell (MNC) cultures. MNC were isolated from the blood of different groups of donors: healthy volunteers, patients with Graves' disease (autoimmune hyperthyreosis- AH) prior to treatment and patients after kidney, transplantation receiving immunosuppressive therapy (IST) with Cyclosporin A (CsA) and corticosteroids. MNC in the cultifres were activated with different amounts of mitogenic lectins, concanavalin A (ConA) and phytohemagglutinin (PHA), alone and in combination with phorbol mirystate acetate (PMA). Four modes of MNC stimulation were used in the experiments: ConA, PHA, ConA&PMA with PHA&PMA. At the end of the incubation the concentration of cytokines in cell culture supernatants was estimated by radioimmunoassay (RIA). Results.(trunc.)
Deskriptorji		MONOCYTES INTERLEUKIN-1 INTERLEUKIN-2 CELLS, CULTURED BLOOD DONORS KIDNEY TRANSPLANTATION CYCLOSPORINE HYPERTHYROIDISM CONCANAVALIN A PHYTOHEMAGGLUTININS TETRADECANOYLPHORBOL ACETATE