Avtor/Urednik		Kabaso, Doron; Jorgačevski, Jernej; Costa Calejo, Ana-Isabel; Flašker, Ajda; Guček, Alenka; Kreft, Marko; Zorec, Robert
Naslov		Comparison of unitary exocytic events in pituitary lactotrophs and in astrocytes
Tip		članek
Vol. in št.		Letnik 7
Leto izdaje		2013
ISSN		1662-5102 - Frontiers in cellular neuroscience
Jezik		eng
Abstrakt		In regulated exocytosis the merger between the vesicle and the plasma membranes leads to the formation of an aqueous channel (a fusion-pore), through which vesicular secretions exit into the extracellular space. A fusion pore was thought to be a short-lived intermediate preceding full-fusion of the vesicle and the plasma membranes (full-fusion exocytosis). However, transient exocytic events were also observed, where the fusion-pore opens and closes, repetitively. Here we asked whether there are different discrete states of the open fusion-pore. Unitary exocytic events were recorded by the high-resolution cell-attached patch-clamp method in pituitary lactotrophs and brain astrocytes. We monitored reversible unitary exocytic events, characterized by an on-step, which is followed by an off-step in membrane capacitance (C m ), a parameter linearly related to the membrane area. The results revealed three categories of reversible exocytic events (transient fusion-pore openings), which do not end with the complete integration of the vesicle membrane into the plasma membrane. These were categorized according to the observed differences in the amplitude and sign of the change in the real (Re) parts of the admittance signals: in case I events (Re 0) fusion pores are relatively wide; in case II (Re > 0) and case III (Re < 0) events fusion pores are relatively narrow. We show that case III events are more likely to occur for small vesicles, whereas, case II events are more likely to occur for larger vesicles. Case III events were considerably more frequent in astrocytes than in lactotrophs.
Proste vsebinske oznake		astrocytes capacitance measurements equivalent circuit astrociti meritve kapacitivnosti nadomestno vezje