Avtor/Urednik     Grosek, Štefan; Petrin, Živa; Kopitar, Andreja Nataša; Grosek, Jan; Eržen, Janez; Stopar, Tanja; Petreska, Mirjana; Primožič, Janez; Ihan, Alojz
Naslov     Low CD8 T cells in neonates and infants prior to surgery, and health-care-associated infections
Tip     članek
Leto izdaje     2013
ISSN     1328-8067 - Pediatrics international : official journal of the Japan Pediatric Society
Jezik     eng
Abstrakt     Background: Major surgery suppresses the cell-mediated immune response in children and adults. Data on preoperative and postoperative T-cell counts in pediatric surgical patients and their relationship to health-care-associated infection (HAI) are not yet known. Methods: A prospective observational study was carried out in a level III multidisciplinary neonatal and pediatric intensive care unit. Before and after, and in the first 3 days after surgery, lymphocyte subsets in peripheral blood were measured in 28 neonates and infants on flow cytometry. HAI were classified according to CDC/NHSN criteria. Results: Six out of 28 neonates and infants (21.4%) developed HAI (group I-HAI), while 22 out of 28 (78.6%) remained infection free (group II-non-HAI). In group I with HAI, the preoperative median cytotoxic T-lymphocyte (CD8-T-cell) level was found to be below normal, and remained very low throughout the study period. In addition, the median and interquartile CD8 T-cell range (358 cells/mL; 304424 cells/mL) were twice as low compared to group II without HAI (822 cells/mL; 522933 cells/mL; P = 0.013). No differences were found between the two groups with regard to patient demographics and clinical data. Conclusion: Neonates and infants who underwent a major surgical procedure and who had a very low preoperative CD8 T-cell level, developed HAI postoperatively.
Deskriptorji     Breast feeding
Proste vsebinske oznake     cell-mediated immunity
children
critical care
celična imunost
otroci
intenzivna nega