Avtor/Urednik		Lipnik-Štangelj, Metoda
Naslov		Flow cytometry analysis of ethanol induced cell death in cultured astrocytes
Tip		članek
Vol. in št.		Letnik 66, št. 8
Leto izdaje		2013
Obseg		str. 1183-1190
ISSN		1310-1331 - Comptes Rendus de L'Academie Bulgare des Sciences
Jezik		eng
Abstrakt		The harmful effects of ethanol in the central nerve system result from direct toxic effects of ethanol as well as indirect effects, involving its metabolites and reactive oxygen substances (ROS). In the brain, astrocytes represent the major cellular localisation of ethanol metabolism; they are crucial in detoxification processes and in the maintenance of homeostasis. It has been found that ethanol can induce apoptosis in culture astrocytes. Since the astrocytes are relatively resistant to apoptosis, we were interested if ethanol is able to induce another form of programmed cell death - necroptosis. Cultured rat cortical astrocytes were used for cell death studies. A cell death was induced by incubation of the cells with different concentrations of ethanol for 24 h. After regeneration period, the cells were stained simultaneously with annexin V-FITC and 7-AAD dye. Different forms of cell death were detected using flow cytometry. We showed that acute exposure of cultured astrocytes to low concentrations (50 mM) of ethanol inhibits apoptosis. On the contrary, the number of necroptotic cells was significantly enhanced. The result is important, because the inhibition of apoptosis itself does not mean an inhibition of cell death. Distinction between different forms of regulated cell death is therefore crucial. In this sense, a flow cytometry analysis represents a simple in vitro approach for cell death studies.
Proste vsebinske oznake		ethanol necroptosis apoptosis nekroptoza apoptoza