Avtor/Urednik		Špik, Vesna; Beović, Bojana; Pokorn, Marko; Drole Torkar, Ana; Vidmar, Darja; Papst, Lea; Seme, Katja; Kogoj, Rok; Müller-Premru, Manica
Naslov		Improvement of pneumococcal pneumonia diagnostics by the use of rt-PCR on plasma and respiratory samples.
Tip		članek
Vol. in št.		Letnik 45, št. 10
Leto izdaje		2013
Obseg		str. 731-737
ISSN		0036-5548 - Scandinavian journal of infectious diseases
Jezik		eng
Abstrakt		Background: The aim of the study was to assess the performance of a real-time polymerase chain reaction (rt-PCR) assay on plasma and respiratory samples for the diagnosis of pneumococcal pneumonia. Methods: Three hundred and forty patients (160 children and 180 adults) with community-acquired pneumonia were included prospectively from January 2011 to May 2012. Blood samples were obtained simultaneously for culture and rt-PCR targeting the lytA gene. Respiratory samples were also obtained: nasopharyngeal swab in nearly all patients and sputum or tracheal aspirate when available. Results: Streptococcus pneumoniae was detected in 222 (65%) of 340 patients: 143 (89%) children and 79 (44%) adults. Pneumonia was assigned as definite pneumococcal in 96 (28.2%) of 340 patients, according to S. pneumoniae detected in blood: in 54 (33.8%) children - by rt-PCR in 51 (31.9%) and by culture in 5 (3.1%); and in 42 (23.3%) adults - by rt-PCR in 41 (22.8%) and by culture in 12 (6.7%). Pneumonia was considered as probably pneumococcal in 19 (10.6%) adults according to S. pneumoniae detected in sputum/tracheal aspirate, by rt-PCR in 19 and by culture in 5. In 18 adults and 89 children with S. pneumoniae detected only in the nasopharynx, pneumonia was considered as possibly pneumococcal; however it should be noted that nasopharyngeal colonization with S. pneumoniae is also common in children with other aetiologies of pneumonia. Conclusions: rt-PCR on plasma and other samples performed significantly better than culture for the detection of pneumococcal pneumonia (p < 0.0005) in children and adults.
Proste vsebinske oznake		pneumococcal pneumonia children and adults blood and respiratory samples pnevmokokna pljučnica otroci in odrasli vzorci krvi in dihal