Avtor/Urednik		Gubina, M; Radšel-Medvešček, A; Pokrajac, T
Naslov		Hemokultura kot diagnostična metoda
Prevedeni naslov		Blood culture as a diagnostic method
Tip		članek
Vir		Zdrav Vestn
Vol. in št.		Letnik 64, št. 1
Leto izdaje		1995
Obseg		str. 9-12
Jezik		slo
Abstrakt		Background. Blood culture is a diagnostic method which enables isolation of bacteria and fungi from blood and therefore permits the clinical doctor to ultimately determine the cause of sepsis. This article presents the circumstances influencing the success of blood cultivation from blood culture and an overlook of the results of five year period of blood culture sample cultivation. Methods. Blood samples for blood culture were sent to the Institute of Microbiology Medical Faculty for diagnostics from various departments of University Medical Centre Ljubljana. The obtained sterile blood samples were bedside injected in the standart liquid media for blood culture at various departments. Inoculated culture media were incubated for 7 to 10 days in the laboratory. During this period, they were regularly examined and cultured on enriched solid media on the 1st, 3rd and 7th day and incubated in such a conditions that different types of aerobic and anaerobic bacteria and fungi were able to grow. The first information on the growth of microbes in a blood culture sample was obtained in 24 hours after the sample was accepted in the laboratory. Results. In the five year period (1989-1993), 39.364 samples of blood cultures were examined, and 5.165 bacterial strains were isolated (13.1 percent of blood samples were positive). The ratios of isolated bacteria in particular years did not show significant aberrations, but the number of Gram-negative bacilli is slowly decreasing. Conclusions. The success of isolated bacteria as a cause of the sepsis depends on many factors concerning the pati ent, blood culture taking, blood culture media and procedure in the laboratory.
Izvleček		Izhodišča. Hemokultura je diagnostična metoda za izolacijo bakterij in gliv iz krvi, ki omogoča kliniku ugotoviti in dokončno opredeliti povzročitelja sepse. V sestavku prikazujemo okoliščine, ki vplivajo na uspeh kultivacije mikrobov iz hemokultur, in pregled rezultatov kultivacija hemokulturnih vzrocev za obdobje petih let. Metode. Na Inštitutu za mikrobiologijo Medicinske fakultete v Ljubljani smo pregledali vzorce hemokultur, ki smo jih prejeli iz različnih oddelkov Kliničnega centra v Ljubljani. Sterilno odvzete vzorce kvri so na oddelkih vbrizgali v tekoča gojišča za hemokulture. Inokulirana gojišča smo v laboratoriju inkubirali 710 dni. V tem času smo jih redno pregledovali in precepljati 1., 3. in 7. dan na obogatena trdna gojišča in jih inkubirali tako, da so lahko zrasle aerobne in anaerobne bakterije ter glive. Prve informacije o rasti mikrobov v hemokulturnem vzorcu smo ugotovili že v 24 urah po sprejemu vzorca v laboratorij. Rezultati. Skupno smo v petih letih pregledali 39.364 vzorcev hemokultur, iz katerih smo izolirali 5165 bakterijskih sevov, kar je povprečno 13.1 odst. pozitivnih vzorcev. Razmerja med izoliranimi bakterijami v posameznih letih niso kazala izrazitih odstopanj med posameznimi obdobji, a vendar v zadnjih letih nekoliko upada število po Gramu negativnih bacilov. Zaključki. S svojim prispevkom smo želeli prikazati rezultate kultivacije hemokultur pri nas in poudariti, da je uspeh izolacije povzročitelja vročinskega stanja odvisen od mnogih dejavnikov, ki so povezani z bolnikom, odvzemom krvnega vzorca, gojišči in postopkom dela v laboratoriju.
Deskriptorji		BACTEREMIA SEPTICEMIA SPECIMEN HANDLING BACTERIAL INFECTIONS