Avtor/Urednik		Cvetko, Erika; Janáček, Jiří; Kubínová, Lucie; Eržen, Ida
Naslov		The capillary pattern in human masseter muscle duringageing
Tip		članek
Vol. in št.		Letnik 32, št. 3
Leto izdaje		2013
Obseg		str. 135-144
ISSN		1580-3139 - Image Analysis & Stereology online journal (Printed version).
Jezik		eng
Abstrakt		The effect of ageing on the capillary network in skeletal muscles has produced conflicting results in both,human and animals studies. Some of the inconsistencies are due to non-comparable and biased methods thatwere applied on thin transversal sections, especially in muscles with complicated morphological structures,such as in human masseter muscle. We present a new immunohistochemical method for staining capillariesand muscle fibres in 100 m thick sections as well as novel approach to 3D visualization of capillaries andmuscle fibres. Applying confocal microscopy and virtual 3D stereological grids, or tracing capillaries invirtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to muscle fibre perfibre length, fibre surface or fibre volume were evaluated in masseter muscle of young and old subjects byan unbiased approach. Our findings show that anatomic capillarity is well maintained in masseter muscle in oldsubjects; however, vascular remodelling occurs with age, which could be a response to changed musclefunction and age-related muscle fibre type transformations.
Proste vsebinske oznake		3D analysis capillaries confocal microscopy 3D analiza kapilare konfokalna mikroskopija