Avtor/Urednik     Ružić-Sabljić, Eva; Maraspin-Čarman, Vera; Cimperman, Jože; Strle, Franc; Lotrič-Furlan, Stanka; Stupica, Daša; Cerar, Tjaša
Naslov     Comparison of isolation rate of Borrelia burgdorferi sensu lato in two different culture media, MKP and BSK-H
Tip     članek
Vol. in št.     Letnik 20, št. 7
Leto izdaje     2014
Obseg     str. 636-641
ISSN     Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases Med
Jezik     eng
Abstrakt     The aim of the study was to evaluate two culture media for Borrelia burgdorferi sensu lato isolation from 5x2x2 mm skin biopsy that was dissected into two pieces and inoculated into modified Kelly-Pettenkofer (MKP) and Barbour-Stoenner-Kelly-H (BSK-H) medium. Samples were incubated at 33 °C for up to 9 weeks. Borrelia species was determined by MluI-restriction of whole genome or by MseI-restriction of PCR product. We determined the proportion of isolation rate, slow-growers, contaminated specimens, and Borrelia species in the two media. In each of the two media 235 skin specimens were cultivated. We found 90/470 (19.1 %) contaminated cultures (BSK-H 67/235, 28.5 %; MKP 23/235, 9.8 %; (p<0.0001). Borrelia growth was ascertained in 59/235 (25.1 %) BSK-H and 102/235 (43.4 %) MKP cultures (p<0.0001); the corresponding values for non-contaminated cultures were 59/168 (35.1 %) and 102/212 (48.1 %); (p=0.003). Fourteen specimens were positive only in BSK-H, 57 solely in MKP, and 43 in both culture media. Slow growth was present in 8/59 (13.6 %) BSK-H and in 4/98 (4.1 %) MKP positive cultures (p=0.019). B. afzelii was identified in 44/51 (86.3 %) BSK-H and in 88/98 (89.8 %) MKP culture positive samples; the corresponding findings for B. garinii and B. burgdorferi sensu stricto were 6/51 (11.8 %) and 9/98 (9.2 %), and 1/51 (1.9 %) and 1/98 (1.0 %), for BSK-H and MKP, respectively. Comparison of MKP and BSK-H medium for Borrelia culturing from skin specimens of European patients with erythema migrans revealed the advantage of MKP over BSK-H.
Proste vsebinske oznake     Borrelia burgdorferi sensu lato
isolation rate
culture medium