Avtor/Urednik		Hudnik-Plevnik, T; Črešnar, B
Naslov		Factors affecting the induction of 11 alpha-hydroxylase of progesterone in the filamentous fungus Rhizopus nigricans
Tip		članek
Vir		J Steroid Biochem
Vol. in št.		Letnik 35, št. 6
Leto izdaje		1990
Obseg		str. 749-51
Jezik		eng
Abstrakt		The 11 alpha-hydroxylase of progesterone was induced in the filamentous fungus Rhizopus nigricans ATCC 6227b with different steroids as inducers and the induction process was optimized in regard to the age of the mycelium, to the concentration of the inducer and to the time of induction. Deoxycorticosterone and testosterone, steroids with higher polarity of the side-chain than progesterone, although poorer substrates for in vivo hydroxylation than progesterone, induced more enzyme compared to progesterone. Other alterations in the steroidal ring system examined diminished the induction capability of the inducing steroid to different extent. The highest 11 alpha-hydroxylating activity, if expressed on the basis of mycelial wet weight, was achieved with 18 h old mycelium which was induced for 2 h with 0.30 mM deoxycorticosterone.
Deskriptorji		PREGNANES RHIZOPUS SEX HORMONES STEROID HYDROXYLASES ENZYME INDUCTION RHIZOPUS SPECIES SPECIFICITY TIME FACTORS