Avtor/Urednik     Gulati, J; Akella, AB; Nikolić, SD; Starc, V; Siri, F
Naslov     Shifts in contractile regulatory protein subunits troponin T and troponin I in cardiac hypertrophy
Tip     članek
Vir     Biochem Biophys Res Commun
Vol. in št.     Letnik 202, št. 1
Leto izdaje     1994
Obseg     str. 384-90
Jezik     eng
Abstrakt     To examine the molecular basis of hypertrophied heart failure, we investigated the changes in cardiac contractile regulatory proteins. The guinea pigs were subjected to chronic pressure overload with aortic banding to induce ventricular hypertrophy, and in-situ pressure-volume relations were recorded together with biochemical characterizations to ascertain the contractile modifications. Immunoblots of left and right ventricular samples revealed four distinct troponin T isoforms, which underwent alterations during hypertrophy. The higher molecular weight bands TnT1 and TnT2 shifted towards the lower molecular weight isoforms TnT3 and TnT4. For TnI, a single prominent band was detected, whose intensity also increased with pump failure. The findings provide the first direct evidence of TnT and TnI shifts in an experimentally induced hypertrophied heart failure and has novel mechanistic implications for the future studies.
Deskriptorji     HEART
HEART HYPERTROPHY
MYOCARDIUM
TROPONIN
BIOLOGICAL MARKERS
BLOTTING, WESTERN
DIASTOLE
ELECTROPHORESIS, POLYACRYLAMIDE GEL
GUINEA PIGS
HEART
HEART HYPERTROPHY
MACROMOLECULAR SYSTEMS
MYOCARDIAL CONTRACTION
REFERENCE VALUES
SYSTOLE
TROPONIN
VENTRICULAR FUNCTION, LEFT