Avtor/Urednik		Lefrancois, D; Kokalj, N; Viegas-Pequignot, E; Montagnier, L; Dutrillaux, B
Naslov		High recurrence of rearrangements involving chromosome 14 in an ataxia telangiectasia lymphoblastoid cell line and in its mutagen-treated derivatives
Tip		članek
Vir		Hum Genet
Vol. in št.		Letnik 86, št. 5
Leto izdaje		1991
Obseg		str. 475-80
Jezik		eng
Abstrakt		The cytogenetic characterization of CH cell line obtained by Epstein-Barr-virus transformation of the lymphocytes of a patient affected by ataxia telangiectasia is reported. Control CH cells and 2 subcultures treated with the mutagens R7000 or NQO were developed in parallel and studied. A common chromosome anomaly, a der(14) t(11;14) (q13.2;q32), was found in all the studied karyotypes, indicating that it occurred either in vivo or early in vitro. In non-treated cultures, additional anomalies were present in 6 derived subclones. All R-7000 treated cells had the same karyotype corresponding to one of the subclones observed without prior treatment. All NQO-treated cells acquired 2 common anomalies, and could be differentiated into 2 subclones because of the addition of a t(7;14) or a t(11;14). Chromosome 14 was involved in various rearrangements after breakage in band q11.2 or q12 in 6/8 subclones. This was not correlated with tumorigenicity, which was clearly increased in mutagen-treated cells as tested by in vitro growth in semi-solid medium and in vivo by grafts into nude mice or growth on the chorio-allantoic membrane of chick embryos. The CH cell line and its derivatives appear to be a promising in vitro system, showing various stages progressing towards malignancy, and reproducing a number of chromosome anomalies spontaneously occurring in AT patients.
Deskriptorji		ATAXIA TELANGIECTASIA CHROMOSOME ABERRATIONS CHROMOSOMES, HUMAN, PAIR 14 ADULT ANTIGENS, VIRAL CELL LINE, TRANSFORMED CELL TRANSFORMATION, NEOPLASTIC CELL TRANSFORMATION, VIRAL HERPESVIRUS 4, HUMAN KARYOTYPING MUTAGENS NEOPLASM TRANSPLANTATION NITROFURANS NITROQUINOLINE-1-OXIDE