Avtor/Urednik		Todorović, Vesna; Kamenšek, Urška; Serša, Gregor; Čemažar, Maja
Naslov		Changing electrode orientation, but not pulse polarity, increases the efficacy of gene electrotransfer to tumors in vivo
Tip		članek
Leto izdaje		2014
Obseg		str. [1-9]
Jezik		eng
Abstrakt		Gene electrotransfer is an established method for plasmid delivery into different tissues. Contrary to extensivein vitro studies demonstrating increased gene electrotransfer by changing the electric field direction during thepulse delivery, little is known about the efficiency of both polarities pulses in vivo. Therefore the aim of ourstudywas to evaluate the effect of pulse polarity and orientation on the efficacy of gene electrotransfer in themurinefibrosarcoma tumor model by using the luciferase and GFP reporter gene expression plasmids. Our resultsdemonstrated no significant difference in luciferase activity, GFP transfected area or fluorescence intensity betweendifferent sets of electric pulses. Inversion of the pulse polarity did not result in the increase of gene transfer,but non-significant enhancement up to 7-fold was detected by changing the electric field orientation in perpendiculardirection. Also, transfection of surrounding skin tissue was observed, meaning that intratumoral geneelectrotransfer could also result in a systemic effect. Our data indicate that testedmodifications of electric pulsesare not significantly affecting the efficiency of gene electrotransfer to LPB tumors. Biological factors, like tissuecomposition, might be of greater importance and should therefore also be taken into account when selectingthe electric pulse parameters for specific tissues.
Proste vsebinske oznake		electroporation gene electrotransfer murine fibrosarcoma pulse polarity