Avtor/Urednik		Kulkarni, Mukta Vishwanath; Flašker, Ajda; Lokar, Maruša; Mrak Poljšak, Katjuša; Mazare, Anca; Artenjak, Andrej; Čučnik, Saša; Kralj, Slavko; Velikonja, Aljaž; Schmuki, Patrik; Kralj-Iglič, Veronika; Sodin-Šemrl, Snežna; Iglič, Aleš
Naslov		Binding of plasma proteins to titanium dioxide nanotubes with different diameters
Tip		članek
Vol. in št.		Letnik 10
Leto izdaje		2015
Obseg		str. 1359-1373
ISSN		1178-2013 - International journal of nanomedicine
Jezik		eng
Abstrakt		Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO2) nanotubes (NTs) by electrochemical anodization. The zeta potential (zeta-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of zeta-potential on salt concentration, whereby the absolute value of zeta-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considering the NT lengths) is available for 100 nm NTs, with decreasing surface area for 50 and 15 nm NTs. These current investigations will have an impact on increasing the binding ability of biomedical devices in the body leading to increased durability of biomedical devices.
Proste vsebinske oznake		protein binding serum amyloid A beta2-glycoprotein I immunoglobulin G histone HA