Avtor/Urednik		Turk, T; Maček, P; Šuput, D
Naslov		Inhibition of acetylcholinesterase by a pseudozoanthoxanthin-like compound isolated from the zoanthid Parazoanthus axinellae (O. Schmidt)
Tip		članek
Vir		Toxicon
Vol. in št.		Letnik 33, št. 2
Leto izdaje		1995
Obseg		str. 133-42
Jezik		eng
Abstrakt		An ethanolic extract from a zoanthid crust coral Parazoanthus axinellae was lethal to mice and crabs and exhibited anticholinesterase activity. The isolation of several AChE inhibitors with the aid of RP-HPLC is described. The most abundant of the inhibitors present in the P.axinellae extract was identified as pseudozoanthoxanthin or an almost identical compound which belongs to the chemically well-characterized series of tetrazacyclopentazulene natural pigments from the genera Parazoanthus, Epizoanthus, Zoanthus and Palythoa. The inhibitor has a mol. wt of 242 and acts as a competitive inhibitor with a Ki of K micro M. The inhibitor exhibited a strong blue fluorescence. In vivo action of crude extract and the isolated inhibitor showed a typical picture of systemic acetylcholinesterase inhibition. Atropinization of experimental animals prior to injection of the inhibitor almost entirely neutralized its activity.
Deskriptorji		CHOLINESTERASE INHIBITORS PIGMENTS MICE RABBITS RATS CHROMATOGRAPHY SPECTROMETRY, FLUORESCENCE ELECTROCARDIOGRAPHY ANESTHESIA, INTRAVENOUS RESPIRATION HEART RATE BLOOD PRESSURE ATROPINE