Avtor/Urednik		Švent-Kučina, Nataša; Pirš, Mateja; Kofol, Romina; Blagus, Rok; Smrke, Dragica; Bilban, Marjan; Seme, Katja
Naslov		Molecular characterization of Staphylococcus aureus isolates from skin and soft tissue infections samples and healthy carriers in the Central Slovenia region
Tip		članek
Leto izdaje		2016
ISSN		0903-4641 - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Jezik		eng
Abstrakt		Staphylococcus aureus is among the most important human pathogens. It is associated with different infections and is a major cause of skin and soft tissue infections (SSTIs). The aim of our study was to compare S. aureus isolates associated with SSTIs with isolates obtained from healthy carriers in the Central Slovenia region in terms of antimicrobial susceptibility, genetic diversity by clonal complex (CC)/sequence type, spa type, and by toxin gene profiling. In total, 274 S. aureus isolates were collected prospectively by culturing wound samples from 461 SSTI patients and nasal samples from 451 healthy carriers. We have demonstrated high heterogeneity in terms of CCs and spa type in both groups of isolates. The main clone among SSTI strains was Panton-Valentine leukocidin gene (pvl) positive CC121, whereas the main clone among carrier strains was CC45 carrying a large range of toxin genes. The main spa type in both groups was t091. Pvl was more frequently present in SSTI strains (31.2% SSTI vs 3.6% carrier strains) and staphylococcal enterotoxin C was more frequently present in carrier strains (1.6% SSTI vs 17.0% carrier strains). We have also demonstrated that methicillin-resistant S. aureus was a rare cause (2.8%) of SSTIs in our region.
Proste vsebinske oznake		skin and soft tissue infections Staphylococcus aureus molecular characterization okužba kože in mehkih tkiv Staphylococcus aureus molekularna karakterizacija