Avtor/Urednik		Anderluh, G; Pungerčar, J; Štrukelj, B; Maček, P; Gubenšek, F
Naslov		The coding region of the Equinatoxin II gene lacks introns
Tip		članek
Vir		Croat Chem Acta
Vol. in št.		Letnik 68, št. 3
Leto izdaje		1995
Obseg		str. 533-42
Jezik		eng
Abstrakt		Sea anemones produce several toxic peptides and proteins. Equnatoxins (Eqt) isolated from the sea anemone Actinia equina are basic cytolytic proteins with molecular masses of aproximately 20 kDa. Of the three Eqt purified so far, EqtII is the most abudant and well characterized. Its gene organization has not yet been studied. In order to obtain the first information about the EqtII gene structure and sequence, genomic DNA was isolated from A. equina and the target DNA fragment amplified by the polymerase chain reaction (PCR) using three different pairs of oligonucleotide primers deduced from the preserved regions of Eqt cDNA clones. The sequence of the PCR product obtained after amplification of genomic DNA, using an oligonucleotide specific for EqtII, was almost indistinguishable from that of EqtII cDNA. As the DNA fragments derived from PCR reactions preformed of an A. equina cDNA library and EqtII cDNA gene was proved to be intronless, at least within the amplified preproprotein region. The presence of such an intronless gene coding for this cytotoxic protein might be explined by the relative low position of cnidarians in the evolutionary tree or the advantage provided by a potentially higher rate of gene expression.
Deskriptorji		SEQUENCE ANALYSIS, DNA NEUROTOXINS POLYMERASE CHAIN REACTION CNIDARIAN VENOMS BASE SEQUENCE SEA ANEMONES