Avtor/Urednik		Aragon-Ortiz, F; Brenes-Brenes, JR; Gubenšek, F
Naslov		Characterization of a lectine-like protein isolated from Lachesis muta snake venon
Tip		članek
Vir		Rev Biol Trop
Vol. in št.		Letnik 37, št. 1
Leto izdaje		1989
Obseg		str. 79-84
Jezik		eng
Abstrakt		A lecitin-like protein was isolated from L. muta venom by gel filtration on BIO Gel P-100 followed by column Chromatography on DEAE-Sephadex A-50. The protein eluted from 0.4 M NaCl in the 0.01 Tris pH 7.3 and exhibited agglutinin activity toward 0+ human erythrocytes. The protein is a dimer with Mr 28 kDa. Amino acid analysis revealed high content tryptophan and acid recidues and low content of cysteine and methoionine residues. No neutral carbohydrates and sialic acid were detected. Circular dichroic spectrum shows 78 percent of B structure and 1 percent of alfa structure. In vitro experiments with ery throcytes from rat, rabbit and dog revealed strong aggltination while red blood cells from mice, sheep and goat were not agglutinated. In vivo exeriments using non-anesthetized rats, a sharp and prolonged fall in the blood pressure was observed at protein dose of 1.5 mg/kg. Double dose of protein caused the death of the animal.
Deskriptorji		SNAKE VENOMS LECTINS CHROMATOGRAPHY, GEL CIRCULAR DICHROISM BLOOD PRESSURE ERYTHROCYTES AGGLUTINATION