Avtor/Urednik		Smerdu, V; Karsch-Mizrachi, I; Campione, M; Leinwand, L; Schiaffino, S
Naslov		Type IIx myosin heavy chain transcripts are expressed in type IIb fibers of human skeletal muscle
Tip		članek
Vir		Am J Physiol
Vol. in št.		Letnik 267
Leto izdaje		1994
Obseg		str. 1723-8
Jezik		eng
Abstrakt		Type IIx myosin heavy chain transcripts are expressed in type IIb fibers of human skeletal muscle. Am. J. Physiol. 267 (Cell Physiol. 36): C1723-C1728, 1994. Several members of the sacromeric myosin heavy chain (MHC) gene family have been mapped in the human genome but many of them have not yet been identified. In this study we report the identification of two human skeletal MHC genes as fast IIc and IIx MHC based on pattern of expression and sequence homology with the corresponding rat genes in the 3'-translated and untranslated regions. The distributions of these two gene products as well as that of the beta/slow MHC gene was analyzed in the human skeletal muscles by in situ hybridization. The distribution of beta/slow, IIa, and IIx MHC transcripts defines three major muscle fiber types expressing a single MHC mRNA, i.e. either beta/slow, IIa, or IIx MHC mRNA, and two populations of hybrid fibres coexpressing beta/slow with IIa or IIa with IIx MHC mRNA. Fiber typing by ATPase histochemistry shows that IIa MHC transcripts are more abudant in the histochemical type IIa fibers, whereas IIx MHC transcripts are more abudant in the hisdtocemical type IIb fibres.
Deskriptorji		MUSCLE, SKELETAL MUSCLE FIBERS, FAST-TWITCH GENES CHILD ADOLESCENCE ADULT MIDDLE AGE AGED RATS HISTOCYTOCHEMISTRY MYOSIN ATPASE IN SITU HYBRIDIZATION