Avtor/Urednik     Premrov Bajuk, Blanka; Furlan, Tjaša; Černelč, Peter; Čeh, Marija; Podgornik, Helena
Naslov     Monoclonal B-cell lymphocytosis in the population of Slovenian region of Lower Carniola
Tip     članek
Vol. in št.     Letnik 38, št. 4
Leto izdaje     2016
Obseg     str. 341-346
ISSN     International journal of laboratory hematology Med
Jezik     eng
Abstrakt     Introduction: Monoclonal B-cell lymphocytosis (MBL) is a premalig- nant asymptomatic condition characterized by the presence of a small population of monoclonal B lymphocytes (less than 5 9 109/L) in the venous blood of otherwise healthy individuals. Incidence of MBL is higher among first-degree relatives of patients with chronic lympho-cytic leukemia (CLL) and also increases with age and among men. We compared the incidence of MBL found in the same environment in both an open general population and relatives of CLL patients. Methods: Samples were analyzed with 5-parameter flow cytometry using following monoclonal antibodies: kappa and lambda clonality, CD5, CD19, CD20. We analyzed 216 samples of patients in the Slovenian area of Lower Carniola aged 23-88 years. Fifty-four patients who came from families where CLL is present were com- pared by age and gender to 162 healthy individuals from the general population of the same region. Results: We found 10 cases of MBL (4.6%). In 9.4% of younger (% 50 years) first-degree relatives of CLL patients with immunophe- notypic characteristics of atypical MBL, we observed a prevailingly normal j to k ratio. The difference in incidence of MBL cases between relatives and the general population decreases with age. Conclusion: MBL in Slovenia can be coincidentally found with a similar frequency to previously studied populations of other parts of Europe. We found, however, a higher incidence of atypical MBL among first-degree relatives.
Proste vsebinske oznake     Monoclonal B-cell lymphocytosis
chronic lymphocytic leukemia
flow cytometry