Avtor/Urednik		Kos, J; Dolinar, M; Turk, V
Naslov		Isolation and characterisation of chicken L- and H-kininogens and their interaction with chicken cysteine proteinases and papain
Tip		članek
Vir		Agents Actions Suppl
Vol. in št.		Letnik 38, št. 1
Leto izdaje		1992
Obseg		str. 331-9
Jezik		eng
Abstrakt		We have isolated L- and H-kininogens from chicken egg white and plasma using affinity chromatography on CM-papain Sepharose, Con-A Sepharose, gel filtration and ion exchange chromatography, in pure form. In chicken plasma mostly HK was present, whereas in chicken egg white both LK and HK were identified. The determined Mr of LK and HK were 69000 and about 96000, respectively. They occurred in multiple forms with pI 4.3-5.2. The inhibitory activity of chicken HK was tested against cysteine proteinases cathepsins B and L, isolated from chicken liver, and papain. The obtained Ki values demonstrated that chicken H-kininogen is a strong inhibitor of chicken cathepsin L and papain, but much weaker inhibitor of chicken cathepsin B.
Deskriptorji		CHICKENS KININOGENS AMINO ACID SEQUENCE CATHEPSIN B CATHEPSINS CHICK EMBRYO CHROMATOGRAPHY CYSTEINE PROTEINASE INHIBITORS EGG PROTEINS KINETICS KININOGENS MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT PAPAIN