Avtor/Urednik		Žerovnik, E; Kos, J; Lenarčič, B; Turk, V
Naslov		Refolding of chicken cystatin and human stefin A studied by fast protein liquid chromatography (FPLC)
Tip		članek
Vir		Biol Chem Hoppe Seyler
Vol. in št.		Letnik 371, št. Suppl
Leto izdaje		1990
Obseg		str. 151-6
Jezik		eng
Abstrakt		Denaturant gradient gel-chromatography ŠEndo, S. et al. (1983) Anal. Biochem. 131, 108-120Ć has been used to follow renaturation of chicken cystatin and human stefin A. Slow gradient of GuHCl was applied to the size-exclusion Superose 12 column. The column was maintained at 5 degrees C by circulating water from a cryostate. When the denatured protein has been injected it started to refold on the column. By the simplified equation, according to ŠHanai, R. et al. (1986) Biophys. Chem. 25, 27-36Ć approximate kinetics of folding was obtained. No intermediate species in folding could be detected from 6M to 2M GuHCl, only the close-to-denatured state and the close-to-native state.
Deskriptorji		CYSTATINS CHICKENS CHROMATOGRAPHY, GEL CHROMATOGRAPHY, HIGH PRESSURE LIQUID KINETICS PROTEIN CONFORMATION PROTEIN DENATURATION TEMPERATURE