Avtor/Urednik		Lenarčič, B; Ritonja, A; Turk, B; Dolenc, I; Turk, V
Naslov		Characterization and structure of pineapple stem inhibitor of cysteine proteinases
Tip		članek
Vir		Biol Chem Hoppe Seyler
Vol. in št.		Letnik 373, št. 7
Leto izdaje		1992
Obseg		str. 459-64
Jezik		eng
Abstrakt		The complete amino acid sequence of the inhibitor of cysteine proteinases from pineapple stem acetone powder was determined. The inhibitor consists of 52 amino acids and is composed of two polypeptide chains (41 and 11 amino acids) linked via disulphide bonds. It differs from already known sequences in one to four amino acids. Data from its amino acid sequence analysis clearly show that this inhibitor cannot be a member of the cystatin superfamily. The Ki values for papain, bromelain and cathepsin L were determined.
Deskriptorji		CYSTEINE PROTEINASE INHIBITORS PLANTS AMINO ACID SEQUENCE CHROMATOGRAPHY, DEAE-CELLULOSE CHROMATOGRAPHY, HIGH PRESSURE LIQUID CYSTATINS CYSTEINE PROTEINASE INHIBITORS KINETICS MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT X-RAY DIFFRACTION