Avtor/Urednik		Jorgačevski, Jernej; Potokar, Maja; Kreft, Marko; Guček, Alenka; Mothet, Jean-Pierre; Zorec, Robert
Naslov		Astrocytic vesicle-based exocytosis in cultures and acutely isolated hippocampal rodent slices
Tip		članek
Leto izdaje		2017
Obseg		str. str.
ISSN		0360-4012 - Journal of neuroscience research
Jezik		eng
Abstrakt		Astrocytes are excitable neural cells that contribute to brain information processing via bidirectional communication with neurons. This involves the release of gliosignaling molecules that affect synapses patterning and activity. Mechanisms mediating the release of these molecules likely consist of non-vesicular and vesicularbased mechanisms. It is the vesicle-based regulated exocytosis that is an evolutionary more complex process. It is well established that the release of gliosignaling molecules has profound effects on information processing in different brain regions (e.g., hippocampal astrocytes contribute to long-term potentiation [LTP]), which has traditionally been considered as one of the cellular mechanisms underlying learning and memory. However, the paradigm of vesicle-based regulated release of gliosignaling molecules from astrocytes is still far from being unanimously accepted. One of the most important questions is to what extent can the conclusions obtained from cultured astrocytes be translated to in vivo conditions. Here, we overview the properties of vesicle mobility and their fusion with the plasma membrane in cultured astrocytes and compare these parameters to those recorded in astrocytes from acute brain hippocampal slices. The results from both experimental models are similar, which validates experiments on isolated astrocytes and further supports arguments in favor of in vivo vesicle-based exocytotic release of gliosignaling molecules.
Proste vsebinske oznake		astrocytes brain slices exocytosis astrociti možganske rezine eksocitoza