Avtor/Urednik		Korošec, Peter; Turner, Paul J.; Šilar, Mira; Kopač, Peter; Košnik, Mitja; Gibbs, Bernhard F.; Shamji, Mohamed H.; Custovic, Adnan; Rijavec, Matija
Naslov		Basophils, high-affinity IgE receptors, and CCL2 in human anaphylaxis
Tip		članek
Leto izdaje		2017
Obseg		str. str.
ISSN		0091-6749 - The Journal of allergy and clinical immunology
Jezik		eng
Abstrakt		Background: The role of basophils in anaphylaxis is unclear. Objective: We sought to investigate whether basophils have an important role in human anaphylaxis. Methods: In an emergency department study we recruited 31 patients with acute anaphylaxis, predominantly to Hymenoptera venom. We measured expression of basophil activation markers (CD63 and CD203c); the absolute number of circulating basophils; whole-blood FCER1A, carboxypeptidase A3 (CPA3), and L-histidine decarboxylase (HDC) gene expression; and serum markers (CCL2, CCL5, CCL11, IL-3, and thymic stromal lymphopoietin) at 3 time points (ie, during the anaphylactic episode and in convalescent samples 7 and 30 days later). We recruited 134 patients with Hymenoptera allergy and 76 healthy control subjects for comparison. We then investigated whether the changes observed during venom-related anaphylaxis also occur during allergic reactions to food in 22 patients with peanut allergy undergoing double-blind, placebo-controlled food challenge to peanut. Results: The number of circulating basophils was significantly lower during anaphylaxis (median, 3.5 cells/[micro]L) than 7 and 30 days later (17.5 and 24.7 cells/[micro]L, P < .0001) and compared with those in patients with venom allergy and healthy control subjects (21 and 23.4 cells/[micro]L, P < .0001). FCER1A expression during anaphylaxis was also significantly lower than in convalescent samples (P <- .002) and control subjects with venom allergy (P < .0001). CCL2 levels (but not those of other serum markers) were significantly higher during anaphylaxis (median, 658 pg/mL) than in convalescent samples (314 and 311 pg/mL at 7 and 30 days, P < .001). Peanut-induced allergic reactions resulted in a significant decrease in circulating basophil counts compared with those in prechallenge samples (P = .016), a decrease in FCER1A expression (P = .007), and an increase in CCL2 levels (P = .003). Conclusions: Our findings imply an important and specific role for basophils in the pathophysiology of human anaphylaxis.
Proste vsebinske oznake		anaphylaxis basophils serum tryptase anafilaksa bazofili serumska triptaza