Avtor/Urednik		Rotter, A
Naslov		Določanje Borreliae burgdorferi v tkivu z imunofluorescentno metodo
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1994
Obseg		str. 98
Jezik		slo
Abstrakt		Borrelia burgdorferi (BB), the causative agent of Lyme disease, was isolated in the year 1981 (1) in spite the fact Lenhoff claimed that he found spirochaetes in skin biopsies of Erythema Chronicum Migrans (ECM) as early as 1948 (2). Rountinely, skin biopsies suspected of borrelial infection, are contrasted with the silver-staining method (3, 4) in order to discover spirochaetes. Unfortunately, tissue structures are often silver stained, as well, making any present spirochaetes invisible or unrecognisable. On the less stained tissue slices there might be only a very slight contrast between the tissue and the spirochaetes, so the spirochaetes are simply overlooked. Because of difficulties with silver-staining the goal of our work was to introduce a so-called "inverted" indirect immunofluorescent method (OIIF), based on a specific immune reaction between the antigen (BB) and the antibody in the tissue. We call the OIIF method "inverted" because of the known IgG or IgG/IgM antibodies against BB provided by infected patients and used to mark the BB spirochaetes as the antigen in the tissue. In our work we used 143 biopsies which had been collected over a period of four years (1990093), and which were routinely checked before the study. In spite of its being a new, experimental method, in five cases, spirochaetes were clearly seen with the OIIF method. In ten cases, spirochaetes were clearly seen with the routin silver-staining method. The positive cases didn't overlap, meaning that we couldn't overlap, meaning that we couldn't compare the sensitivity of both methods on human ma terial because it was imposibble to use both methods on the same tissue speciumen.(trunc.)
Deskriptorji		LYME DISEASE BORRELIA BURGDORFERI FLUORESCENT ANTIBODY TECHNIQUE BIOPSY SILVER STAINING ANIMALS, LABORATORY RABBITS IGG IGM ANTIBODIES, ANTI-IDIOTYPIC SKIN