Avtor/Urednik     Terčelj-Zorman, Marjeta; Salobir, Barbara; Rylander, Ragnar
Naslov     [Beta]-glucan in the lymph nodes in sarcoidosis and in Kveim-Siltzbach test reagent
Tip     članek
Vol. in št.     , št. Vol. 34
Leto izdaje     2017
Obseg     str. 130-135
ISSN     1124-0490 - Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders
Jezik     eng
Abstrakt     Background: Previous studies have demonstrated a relationship between biomass of fungi exposure in the home and the risk of sarcoidosis. [beta]-glucan was present in the bronchial alveolar lavage fluid (BALF) of patients with sarcoidosis. The Kveim-Siltzbach test reagent (KSTR) induces a sarcoidosis specific, granulomatous, cutaneous response and was used to establish the diagnosis. To date, the granuloma-inducing component of KSTR is still unknown. The present study was undertaken to investigate the presence of [beta]-glucan in the lymph nodes of patients with sarcoidosis and to determine the relationship between the amounts of this agent with disease severity and to investigate the presence of [beta]-glucan in KSTR. Materials and methods: Lymph node aspirations were collected by transbronchial needle aspiration (TBNA) in region R4 or 7 from patients with newly diagnosed sarcoidosis. The samples were treated to isolate [beta]-glucan and analyzed using a Limulus-based assay. Cultures of Propionibacterium ac. and Mycobacterium gordonae as well as samples of Kveim-Siltzbach test reagent were analyzed to determine [beta]-glucan content. Results: A significant relationship was observed between the amount of the [beta]-glucan in the lymph nodes and the extent of granuloma formation in the lung parenchyma, and the size of the lymph nodes in the mediastinum (r=0.787, p=0.0001 and r=0.664, p<0.001 respectively, Spearman's test). The samples of Kveim-Siltzbach test reagent contained high levels of [beta]-glucan. Cultures of Propionibacterium ac. and Mycobacterium gordonae contained [beta]-glucan, the levels of which were lower in the Mycobacterium cultures. Comments: The results support the hypothesis that [beta]-glucan, and thus fungal exposure, are involved in the pathogenesis of sarcoidosis.
Proste vsebinske oznake     sarcoidosis
[beta]-glucan
lymph nodes
sarkoidoza
beta glukan
bezgavke