Avtor/Urednik		Goličnik, Marko
Naslov		Closed-form solution of the reduced haldane equation for enzyme kinetics with strong substrate inhibition
Tip		članek
Vol. in št.		Letnik 79, št. 3
Leto izdaje		2018
Obseg		str. 607-618
ISSN		0340-6253 - Match (Mulheim an der Ruhr, Germany)
Jezik		eng
Abstrakt		The Haldane equation has been widely used to describe enzyme kinetics of inhibitory substrates, although its use is limited because the integrated form cannot be expressed in an explicit closedform reformulation of the time-dependent solution. However, the standard three-parameter Haldane rate equation must be per se reduced to describe the kinetics of enzyme-catalyzed reactions that refers to a strongly inhibiting substrate, while the analysis of initial rates instead of complete progress curves over time leads to an additional loss of information relating to the reaction kinetics. Thus, I present an explicit solution of the parameterized two-parameter Haldane equation which is expressed in terms of the Lambert W function, as the most elegant and ideal simplification of the evaluation of kinetics parameters can be performed from progress curves.
Proste vsebinske oznake		kinetika encimov reducirana haldanska enačba substrati enzyme kinetics reduced haldane equation substrates