Avtor/Urednik		Stupnišek, Mirjana; Kokot, Antonio; Drmić, Domagoj; Hrelec Patrlj, Masa; Zenko Sever, Anita; Kolenc, Danijela; Radic, Bozo; Suran, Jelena; Bojic, Davor; Seiwerth, Sven
Naslov		Pentadecapeptide BPC 157 reduces bleedingand thrombocytopenia after amputation in rats treated with heparin, warfarin, L-NAME and L-arginine
Tip		članek
Vol. in št.		Letnik 10, št. 4
Leto izdaje		2015
Obseg		str. 1-12
ISSN		1932-6203 - PloS one
Jezik		eng
Abstrakt		Background BPC 157 is a stable gastric pentadecapeptide recently implicated with a role in hemostasis.While NO is largely implicated in hemostatic mechanisms, in tail-amputation-models underheparin- and warfarin-administration, both the NO-synthase (NOS)-blocker, L-NAME (pro-thrombotic) and the NOS-substrate L-arginine (antithrombotic), were little investigated. Objective. To investigate the effect of L-NAME and L-arginine on hemostatic parameters, and to reveal the effects of BPC 157 on the L-NAME- and L-arginine-induced hemostatic actionsunder different pathological condition: tail amputation without or with anticoagulants, hepa-rin or warfarin. MethodsTail amputation, and/or i.v.-heparin (10 mg/kg), i.g.-warfarin (1.5 mg/kg/day for 3 days) wereused in rats. Treatment includes BPC 157, L-NAME, L-arginine, per se and their combination.Results After (tail) amputation, with or without i.v.-heparin or i.g.-warfarin, BPC 157 (10[micro]g/kg, 10 ng/kg, i.p., i.v. (heparin), 10[micro]g/kg i.g. (warfarin)) always reduced bleeding time and/or haemorrhage and counteracted thrombocytopenia. As for L-NAME and/or L-arginine, we noted: L-arginine (100 mg/kg i.p.)-rats: more bleeding, less/no thrombocytopenia; L-NAME (5 mg/kg i.p.)-rats: less bleeding (amputation only), but present thrombocytopenia; L-NAME+L-arginine-rats also exhibited thrombocytopenia: L-NAME counteracted L-arginine-increased bleeding, L-arginine did not counteract L-NAME-thrombocytopenia. All animals receiving BPC 157 inaddition (BPC 157[micro]g+L-NAME; BPC 157[micro]g+L-arginine, BPC 157[micro]g+L-NAME+L-arginine), exhibited decreased haemorrhage and markedly counteracted thrombocytopenia. Conclusions L-NAME (thrombocytopenia), L-arginine (increased haemorrhage) counteraction and BPC157 (decreased haemorrhage, counteracted thrombocytopenia) with rescue against two different anticoagulants, implicate a BPC 157 modulatory and balancing role with rescued NO-hemostatic mechanisms.
Proste vsebinske oznake		pentadecapeptide thrombocytopenia amputation pentadekapeptid trombocitopenija amputacija