Avtor/Urednik		Filipič, Bratko; Rihar, Klemen; Pereyra Gonzales, Adriana; Deželak, Matjaž; Jaklič, Domen; Kopinč, Rok; Potokar, Jana
Naslov		Antimikrobielle Aktivität von Medex' Zahnpasta mit Propolis "Apident" in vitro
Prevedeni naslov		Antimicrobial activity of Medex' tooth paste with Propolis "Apident" in vitro
Tip		članek
Vol. in št.		Letnik 19Letnik 2
Leto izdaje		2018
Obseg		str. 20-27
ISSN		2628-1325
Jezik		ger
Abstrakt		In this study, we evaluated the antibacterial activity of Medex' toothpaste with Propolis "Apident", used for the treatment of teeth and as a medicine for endodontic treatment. Toothpaste "Apident" was prepared by the Propolis extract (3%) and other main active constituents like: Aloe barbadensis (0.5%) and Sodium fluoride (0.2%). Calcium Hydroxide was a control, as well as two commercial and two medical toothpastes. The bacteria on which "Apident", medical and commerical toothpaste works are: MRSA, Streptococcus mutans, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa and yeast Candida albicans. Inhibition of bacterial / yeast growth was evaluated by colouring with resazurine. The inhibition of bacterial / yeast growth is apparent in changing the colour of resazurine from blue to pink. MIC (Minimum Inhibitory Concentration) was determined as the maximum dilution in which no minimal colour change occurs, or a minimal colour change occurs. For example, in the seventh hole, the colour from pink to blue has changed. Therefore, the colour change in the seventh hole at dilution 1:64 means MIC = 1 X 10/64 = 0.156 mg / ml. MIC values of toothpaste with Propolis were against S. aureus: 0.031 mg / ml (0.326 mg / ml of medical toothpaste, 0.187 mg / ml of commercial toothpaste); Micrococcus luteus: 0.003 mg / ml (0.140 mg / mL of medicinal toothpaste, 0.046 mg / ml of commercial toothpaste); Streptococcus mutans: 0.015 mg / ml (0.140 mg / ml medical toothpaste, 0.019 mg / mL of commercial toothpaste); E. coli: 0.015 mg / ml (0.375 mg / ml medical toothpaste, 0.062 mg / ml of commercial toothpaste); Pseudomonas aeruginosa: 0.062 mg / ml (0.281 mg / ml medical toothpaste, 0.046 mg / ml of commercial toothpaste) and yeast Candida albicans: 0.015 mg / ml (0.140 mg / ml medical toothpaste, 0.281 mg / ml commercial toothpaste ). Comparison of MIC values between toothpaste with Propolis and medical toothpastes demonstrate greater effectiveness of Propolis toothpaste from 4.53 to 25.0 times. For St. aureus, this was 10.51 times; for Str. mutans 9.33 times, E. coli 25.0 times; for Pseudomonas aeruginosa 4.53 times and yeast Candida albicans 9.33 times. Compared with commercial toothpastes, Propolis toothpaste was better: 0.74 to 18.73 times. For St. aureus, was 6.03 times, for Str. mutans 1.26 times, for E. coli 4.13 times; for Pseudomonas aeruginosa 0.74 times and yeast Candida albicans 18.73 times.
Proste vsebinske oznake		zobna pasta Medex protimikrobno delovanje propolis Medex tooth paste antimicrobial activity propolis